Regeln » eyscowboys.com/zack-martin-jersey/

Introduction
Background
The control of microbial growth refers to the inhibiting as well as the prevention of growth of microorganisms. The control of microorganisms can be achieved through killing or inhibiting the growth through the use of physical or chemical agents (Pommerville Wholesale Maliek Collins Jersey , 2014). Agents that kill microorganisms is called cidal agent, whereas, agents that inhibit the growth of the microorganism is called static agent. The control of microbial growth plays significant roles in different industries such as medicine, agriculture as well as food production and preparation.
The physical methods for control of microorganisms aim at achieving sterilization that is the destruction or removal of all life forms such as bacterial spores. Sanitizations entails of techniques that minimize the numbers of or inhibit the growth of microbes. Physical techniques used for the control of microorganisms include heat method, incineration Wholesale Jaylon Smith Jersey , filtration, X-rays and gamma rays, and Ultraviolet (UV) light (Pommerville, 2012). Chemical methods of control of microorganisms are effective. Chemical techniques of control of microorganisms include the use of chemical agents such as phenol, hydrogen peroxide Wholesale Chidobe Awuzie Jersey , formaldehyde and glutaraldehyde, alcohol, halogens, and heavy metals.
Heat is a common approach used in food industry. Heat is common because it is fast, reliable as well as relatively cost-effective. In heat method Wholesale Taco Charlton Jersey , thermal death point and thermal death time get employed to determine the period it takes to kill a population of microbial cells. All microbial species have a thermal death time, which is the time required to kill the population at a specified temperature. All species also have a thermal death point that is the lowest temperature necessary to kill microbes in a given time. Dry heat sterilizers are used only for materials that might get destroyed by moist heat or that are impenetrable to moist heat such as petroleum products, powders, and sharp instruments. Dry heat sterilizers are advantages that include are nontoxic and do not harm the environment; a dry heat cabinet is easy to install and have low operating costs, heat penetrates materials and is noncorrosive for metal and sharp instruments. Dry heat sterilizers are disadvantages because of the rate of heat penetration and thus making the microbial killing method a time-consuming method.
UV radiation is an effective technique of killing microorganisms when used on a dry surface and in a confined air space. The wavelength of UV radiation is between 328 nm and 210 nm. The maximum bactericidal effect takes place at about 240 to 280 nm. Applications of UV radiation include disinfection of drinking water Wholesale Sean Lee Jersey , air, contact lenses and titanium implants. Application of ultraviolet in the health-care environment is limited to the killing of airborne organisms or inactivation of microorganisms on surfaces (Rutala & Weber, 2008).
Hypothesis
The laboratory experiment investigates the effect of temperature in heat method and UV exposure on the growth of bacteria.
Procedures
Effects of Temperature on Bacterial Growth
1) The first phase was acquiring 3 TSA plates
2) The second step was using a labeling pen to divide the plates into half and then labeling each side with the name of a bacterium.
3) The plates were labeled with a name, lab time and temperature.
4) A zig-zag streak of organisms was made on each plate
5) The plates were incubated at a suitable temperature. -35oC incubator, room temperature25oC incubator and refrigerator (~10oC)
Control by UV Light
1) 2 TSA plates were acquired Wholesale Terrance Williams Jersey , in which one plate was inoculated with and the other plate with ilis.
2) The plates were labeled with name, lab time and organism.
3) The bottom of the plate was divided in half using a labeling marker, with one side of the plate labeled control and the other UV exposure.
4) A sterile swab was inoculated into the culture.
5) The organism was plated by making three zig zag lines on the agar surface
6) UV exposure was done at the right conditions and safety measures.
7) The plates were incubated at 35oC for 48 hours
Control by Heat
1) Each participant had 2 Trypticase Soy Broth tubes that were labeled with name, lab time, organism Wholesale Cole Beasley Jersey , temperature and time.
2) The tubes were inoculated with the proper organism
3) Water baths were availed at temperatures 40oC, 55oC, 80oC, and 100oC
4) The organisms were exposed to heat for a given length of time that is 10 minutes, 20 minutes Wholesale Travis Frederick Jersey , 30 minutes and 40 minutes.
5) Team assignments were given in which each team was responsible for organisms at each of the temperature (40oC, 55oC, 80oC and 100oC) at different times.
Result
Effects of Temperature on Bacterial Growth
The presence of growth at the three temperatures was recorded.
The cultures present were drawn, and the colony morphology was described using descriptive factors such as the amount of growth, the shape of the colony Wholesale Zack Martin Jersey , the size of the colony, the color of colony and colony form, elevation and margin.
Control by UV Light
The presence or absence of growth was recorded
Control by Heat